FastQCFastQC Report
Tue 21 Mar 2023
SRR9602596_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9602596_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111048366
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG46441654.182110162701538No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG6664120.6001096855400826No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA3756230.33825171277171245No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC2572780.2316810316686695No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2449250.22055704988941488No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT2223940.20026769236748607No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA1740720.1567533195400642No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCAAAATAGTGGGAAGATT1589590.1431439342385281No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAATCCCAGCACTTTGGGAGG1258670.1133443062097825No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT81488100.0116.836211
GTATCAA83335800.0114.1882259
GGTATCA83877300.0113.5138558
AGTGGTA83922650.0113.423575
GTGGTAT84784100.0112.301686
TGGTATC84834350.0112.255467
AGCAGTG85154650.0111.846342
CAGTGGT85852550.0110.952424
GCAGTGG86577100.0110.020123
ATGGGAG13056950.025.88254225-29
TGACTGG829600.025.8397582
GTGACTG843550.025.4224431
ATGGGAT13036350.025.26322425-29
ATGGGAC7262550.025.20869625-29
CATGGGG17479350.024.72993925-29
ATGGGGT4732400.024.46264525-29
ATGGGGG5121900.024.04353525-29
ACATGGG78941950.023.71457320-24
AGAGTAC81652750.023.55876215-19
TACATGG80720300.023.54397820-24
Produced by FastQC (version 0.11.5)