FastQCFastQC Report
Tue 21 Mar 2023
SRR9602595_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9602595_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109390700
Sequences flagged as poor quality0
Sequence length150
%GC30

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC3226850.29498394287631396No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTATC7587200.0145.497281
AACGTGA8700700.0143.907641
AGAGTCA5785800.0143.808941
CGCATAC3177750.0143.376751
ACGTGAT8790100.0142.525242
GACTAGT1981450.0142.071581
ACGCTTA6703050.0141.909532
CGAACTT4052150.0141.740621
AGTCACT3066350.0141.409621
CGACTGG2367250.0140.804381
GTCACTA3079850.0140.763082
GAGTCAA5904100.0140.74192
AGATCGC1240350.0140.741651
AACATCG4327000.0140.504912
CGTGATG3051600.0140.178093
CGCTTAG2338550.0139.950413
AGCAGGA4992950.0139.76291
GATCGCA1250550.0139.66562
GTATCAG2720200.0139.202273
CCGTGAG1807350.0139.1921
Produced by FastQC (version 0.11.5)