FastQCFastQC Report
Tue 21 Mar 2023
SRR9602595_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9602595_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109390700
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGTAAAGCAAGCAGGAGACGTG48703994.452297133120092No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG18773691.7162053081294846No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG5095980.4658513018017071No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2610800.23866745527727679No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC1997150.18257036475678462No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA1701420.15553607390756252No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCAAAATAGTGGGAAGATT1469710.1343542001285301No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT1460810.1335406026289255No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAATCCCAGCACTTTGGGAGG1430040.1307277492510789No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAGCAAGCAGGAGACGTG1411110.1289972547940547No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT73494200.0131.028231
AGTGGTA74643950.0128.985285
GTATCAA74821150.0128.658899
AGCAGTG74904350.0128.621082
GTGGTAT75015550.0128.367436
GGTATCA75102850.0128.236798
TGGTATC75166750.0128.132867
CAGTGGT75429350.0127.7293854
GCAGTGG76004650.0126.7629553
ACGTATC121150.036.0048751
CATGGGG17437600.026.79362525-29
TGACTGG824700.026.2812042
AGAGTAC74167300.026.19848315-19
ATGGGAG11795550.026.1830925-29
CAGAGTA74259500.026.18076715-19
CAACGCA74700650.026.11979310-14
AGTACAT73979700.026.1176620-24
GCAGAGT74497050.026.0804515-19
GAGACGT6581500.026.07800540-44
TACATGG73820100.026.04187420-24
Produced by FastQC (version 0.11.5)