FastQCFastQC Report
Tue 21 Mar 2023
SRR9602594_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9602594_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103487964
Sequences flagged as poor quality0
Sequence length150
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA15625641.5098992574634091No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC5022730.485344363330986No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA4065700.3928669424784509No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC1902090.18379818545855245No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGA2311400.0144.064211
GATAGAC2316700.0144.019011
TATCAGC6378750.0143.943021
TAGGATG5917100.0141.352191
TAGCGAC2371300.0140.629232
AGAGATC3966950.0140.145692
ATCAGCA6532700.0140.003112
GTCGTAG1299500.0139.93781
GCGAGTA1270450.0139.415421
GATGTAC2709800.0139.364442
TCACGCA3769500.0138.915472
GTGCGAA1458900.0137.028032
ATAGACA2437600.0136.984452
AGTTAGC1593750.0136.634032
GCCACAT3229100.0136.482161
AGGATGA6116150.0136.453632
TTCACGC3860250.0136.263151
GTCTGTC2181600.0135.81151
GGAGAAC3386200.0135.723271
TCAGCAG2310850.0135.642413
Produced by FastQC (version 0.11.5)