FastQCFastQC Report
Tue 21 Mar 2023
SRR9602594_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9602594_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103487964
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG52540745.076990402478108No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA4770800.4610004695811775No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG4414260.4265481539476417No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA3588710.3467755921838408No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2256220.21801762376927233No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT2053230.19840278237573597No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC1752500.1693433644128896No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC1604690.1550605440454892No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA1465890.14164835632479927No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA1462470.1413178831115085No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT78564400.0111.8244861
AGTGGTA80600600.0108.9749455
GTATCAA80934850.0108.494869
AGCAGTG81216850.0108.2125552
GGTATCA81717500.0107.516438
CAGTGGT82030550.0107.148644
GTGGTAT82245650.0106.820736
TGGTATC82346400.0106.71147
GCAGTGG82887500.0106.034163
GTGACTG785050.030.3651451
TGACTGG809400.029.251722
GACTGGA921500.025.8026643
AGTTCAG922850.025.5303069
CATGGGG17695500.024.68663825-29
ATGGGAC6125100.024.56023625-29
ATGGGAT10341350.024.4219525-29
ATGGGAG10985450.024.3108525-29
ATGGGGT4035650.024.15944125-29
ATGGGGG5400250.023.70365325-29
ATGGGGC4226950.023.39031225-29
Produced by FastQC (version 0.11.5)