FastQCFastQC Report
Tue 21 Mar 2023
SRR9602593_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9602593_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76818176
Sequences flagged as poor quality0
Sequence length150
%GC31

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC6773210.8817197117515521No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1281170.16677953925904204No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAGT4532650.0144.725111
ACGTATC3071150.0144.460021
AACGTGA7308950.0143.595181
AGATCGC1821850.0142.970381
GAGCTGA1936350.0142.574161
ACGTGAT7382050.0142.473742
AACCGAG4159900.0141.86151
CGCATAC1799250.0141.857931
AGTCACT2344250.0141.748411
GATCGCA1842050.0141.166212
AGAGTCA1827350.0140.978121
ACCGAGA4143800.0140.92762
CGTGATG2547250.0140.791583
GTCACTA2361950.0140.740842
CGAACTT2145750.0140.423981
ACGCTTA2314650.0140.344252
CCGTGAG1375650.0139.686871
ACTAGTA4711500.0139.226852
CGTGATA2292850.0138.347523
GACGGAT859100.0137.831683
Produced by FastQC (version 0.11.5)