FastQCFastQC Report
Tue 21 Mar 2023
SRR9602593_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9602593_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76818176
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGTAAAGCAAGCAGGAGACGTG41238965.368385732043417No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG34670294.513292531184286No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG3298930.4294465414018682No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA3242180.4220589668778389No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2033370.26469907330265174No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA1481800.1928970560300729No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC1349650.17569409614724515No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT1007770.13118900401904884No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAGCAAGCAGGAGACGTG906910.11805929888259778No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT56390400.0114.019981
AGTGGTA57431850.0111.904895
AGCAGTG57641900.0111.5667652
GTATCAA57916300.0110.931489
CAGTGGT58273450.0110.370514
GGTATCA58473850.0109.925328
GTGGTAT58517250.0109.842286
TGGTATC58579050.0109.747617
GCAGTGG58876700.0109.230833
TGACTGG532850.034.4053082
GTGACTG572050.031.9388121
GACTGGA592900.030.8235193
AGTTCAG623650.029.1334829
ACGTATC81150.025.8408221
CATGGGG11105600.025.61979925-29
ACTGGAG712050.025.603834
GAGACGT5672700.025.41338340-44
TGGAGTT725300.025.140116
AAGACGC5635600.025.04084670-74
CGGTCCT5656750.024.98950460-64
Produced by FastQC (version 0.11.5)