FastQCFastQC Report
Tue 21 Mar 2023
SRR9602592_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9602592_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99845834
Sequences flagged as poor quality0
Sequence length150
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA37462033.7519872887235337No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA9803740.9818877370486985No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC7288950.7300204433166435No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT5268330.527646451428309No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG3038870.30435621380056777No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC2092440.2095670811863818No Hit
CCATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTA1719300.1721954668634447Illumina Single End PCR Primer 1 (100% over 21bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC1053670.10552969090327795No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAGAC5323900.0145.3721
ATAGCGA2238200.0143.757081
GCGAGTA2521400.0143.123051
ATAGACA5508750.0140.146822
TAGCGAC2308650.0138.853972
GTGCGAA1778100.0138.115572
GCCACAT4218800.0138.099291
AGAGATC3102700.0137.831022
ATCCGTC1654200.0137.603972
GTCTGTC1955650.0136.682281
TATCAGC2071400.0136.294451
AGATTCG817600.0135.965553
CGAGTAA2658500.0135.107442
CAGATTC2367450.0134.26472
AGTTAGC1032550.0134.252672
GGTGCGA1838400.0134.064041
AGCGACT805950.0133.768253
GATGTAC1754800.0133.630082
CCGTCTA3420500.0133.515322
ACCTTAC2573650.0132.883182
Produced by FastQC (version 0.11.5)