FastQCFastQC Report
Tue 21 Mar 2023
SRR9602592_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9602592_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99845834
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1049179310.507992752106214No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA5443180.5451584489744459No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG3433730.34390318177922174No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA2045860.20490188904626708No Hit
NAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1576620.15790543649522723No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC1528960.1531320775987509No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA1462920.14651788075604638No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1432900.1435112455468097No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT1257500.12594416307845152No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG1218610.12204915830539309No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT1193840.11956833371735871No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAATCCCAGCACTTTGGGAGG1180710.1182533063923328No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG1112440.11141576522862237No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAG1104780.11064858249368723No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT1041900.10435087356774446No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC1027640.10292267176615501No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT100266300.088.371361
GTATCAA104966650.084.5252159
AGTGGTA105229250.084.284315
GGTATCA106253100.083.49548
AGCAGTG106482400.083.29412
GTGGTAT107912350.082.191036
TGGTATC107953000.082.168287
CAGTGGT107954000.082.165664
GCAGTGG108879450.081.452843
ATGGGAT10588450.024.37148325-29
ATGGGGT4954550.023.66852225-29
ATGGGAG10946500.022.86267525-29
ATGGGAC6527800.022.76497825-29
ATGGGGG4733450.022.55808425-29
ATGGGGC3253500.021.84545725-29
CATGGGG18904350.021.13593525-29
AAACCGC773300.020.96680385-89
TGGGATA2170900.020.79136725-29
GAAACCG799300.020.4252985-89
TGGGATT4182650.020.38088825-29
Produced by FastQC (version 0.11.5)