FastQCFastQC Report
Tue 21 Mar 2023
SRR9602591_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9602591_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108611097
Sequences flagged as poor quality0
Sequence length150
%GC28

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGC3360600.0144.739381
AACGTGA9379950.0144.698171
ACGTATC2215500.0144.396511
CGCATAC3101500.0144.19931
CGAACTT5823850.0143.796171
AGTGGTC5949450.0143.510271
ACGTGAT9529550.0142.528242
GAGCTGA2454000.0142.235261
GATCGCA3418800.0141.932862
AGAGTCA2315850.0140.896131
AAGGTAC5112600.0140.846621
CGACTGG2040300.0140.561041
CGTGATG3317900.0140.549853
GTGGTCA6045650.0140.429922
ACGCTTA2940900.0140.152572
AACATCG3033950.0139.739352
GAACTTA5984100.0139.509842
ATCGCAT1243750.0139.226653
GCATACA3206050.0138.99632
AGGTACA5183750.0138.806092
Produced by FastQC (version 0.11.5)