FastQCFastQC Report
Tue 21 Mar 2023
SRR9602591_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9602591_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108611097
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGTAAAGCAAGCAGGAGACGTG50768394.674328075334696No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG16890351.5551219411769683No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG3585900.3301596336882593No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA2069490.19054130352812843No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC1623230.14945342095200456No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1566300.14421178344234936No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC1426660.1313549019765448No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTATCAACGCAGAGTACATG1318960.12143878815624153No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT70443250.0132.48591
AGTGGTA71857150.0129.975625
AGCAGTG72072150.0129.593372
GTGGTAT72190200.0129.382146
TGGTATC72238350.0129.306587
GTATCAA72280650.0129.271489
GGTATCA72323200.0129.181478
CAGTGGT72355100.0129.096244
GCAGTGG72764950.0128.351753
CATGGGG16995750.026.67393325-29
CAACGCA71910500.026.28612310-14
AGAGTAC71960950.026.2094515-19
CAGAGTA72063250.026.19309815-19
ATCAACG72084550.026.19310-14
AACGCAG72205050.026.17034110-14
CGCAGAG72083000.026.15450915-19
GCAGAGT72118250.026.14907315-19
TCAACGC72297950.026.13619610-14
AGTACAT71942900.026.12814320-24
ATGGGAT11510750.026.08759925-29
Produced by FastQC (version 0.11.5)