FastQCFastQC Report
Tue 21 Mar 2023
SRR9602590_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9602590_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102457193
Sequences flagged as poor quality0
Sequence length150
%GC29

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC1304890.12735953053096039No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGA4261400.0145.751891
GATAGAC2877800.0145.00561
GCGAGTA3327250.0144.096791
TAGCGAC4367300.0142.241712
TGGAACA8553050.0141.636761
GTGCGAA2774150.0140.619052
GTCGTAG1368150.0140.256241
AGAGATC4378800.0140.176032
ACACGAC3072400.0139.996951
GGTGCGA2803350.0139.887081
CCGACAA4249600.0139.88691
TATCAGC1744850.0139.273421
GCCACAT3438300.0139.267521
TCACGCA3531050.0139.244342
ATCCGTC1709600.0139.111482
AGCGACT1539200.0138.95733
AGCCATG1863400.0138.80021
CGACAAC4304850.0138.499272
AGATTCG1014750.0138.288393
GTCTGTC2367850.0138.254381
Produced by FastQC (version 0.11.5)