FastQCFastQC Report
Tue 21 Mar 2023
SRR9602590_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9602590_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102457193
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG7726330.7541032282623632No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA4993200.4873449929474449No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG4060500.3963118528925539No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA1930520.18842210522007957No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1912980.186710170753946No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC1789330.17464171597986294No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAATCCCAGCACTTTGGGAGG1659190.16193982593296305No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA1619090.15802599628119815No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT1331300.1299371924038559No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC1148960.11214049168807505No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCAAAATAGTGGGAAGATT1099730.10733555817794072No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT66896800.0138.068051
AGTGGTA68287750.0135.344645
GGTATCA68366100.0135.235248
GTATCAA68407750.0135.173959
TGGTATC68417150.0135.107067
GTGGTAT68417800.0135.093376
AGCAGTG68494050.0134.927582
CAGTGGT68697350.0134.55434
GCAGTGG69208000.0133.529373
CAACGCA68102200.027.44652610-14
ATCAACG68206650.027.37144510-14
AGAGTAC68217050.027.34217315-19
AACGCAG68364200.027.33038910-14
CAGAGTA68349250.027.30861915-19
CGCAGAG68264500.027.30640415-19
TCAACGC68433850.027.30446610-14
TACATGG67917800.027.29558220-24
AGTACAT68124650.027.29119320-24
GTACATG68065400.027.27889620-24
GCAGAGT68423950.027.2502615-19
Produced by FastQC (version 0.11.5)