FastQCFastQC Report
Tue 21 Mar 2023
SRR9602589_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9602589_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109314757
Sequences flagged as poor quality0
Sequence length150
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC25917032.3708628835903647No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT6407050.5861102540803343No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTGG13045550.0145.039351
ATGCCTA5546100.0143.443011
AGATCGC3793450.0143.06421
GAGCTGA3524350.0142.749181
GACTAGT3259250.0142.6471
AACGTGA6056400.0142.30491
ACGTGAT6092650.0141.644412
CGCATAC2395400.0140.838971
CTGGCAT4683750.0140.719211
GACTGGA13296000.0140.57682
GATCGCA3859650.0140.185072
TGCCTAA5667700.0140.123732
ACGTATC1473700.0139.312241
TGGCATA4730500.0138.967642
ACTTCGA2608600.0138.237752
CGTGATG2171050.0138.166733
AGCTGAA3620100.0137.864932
ACTGGAT4738800.0137.821663
ACTAGTA3370050.0137.248342
CGAAGTA2063650.0137.040072
Produced by FastQC (version 0.11.5)