FastQCFastQC Report
Tue 21 Mar 2023
SRR9602589_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9602589_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109314757
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG102104669.34042784360761No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAAAGCAAGCAGGAGACGTG29938542.7387464256083924No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA6211190.5681931854818101No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG2374750.21723965411184146No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2057260.18819599992341382No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGTAATGAAGAGCGTACGG2040400.18665366470146388No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA1903360.17411738837785642No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG1828760.16729305815499368No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAG1789880.16373635629085284No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA1705760.15604114639343708No Hit
NAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1530930.14004788026926684No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA1319140.12067355187918498No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT1207900.11049743265678211No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT1123020.10273269875173395No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT113189650.094.074111
AGTGGTA116045750.091.829375
AGCAGTG116092600.091.7927252
GTATCAA116935100.091.1650859
CAGTGGT117804050.090.468244
GCAGTGG118531450.089.892293
GGTATCA118705150.089.7933358
GTGGTAT119400600.089.252046
TGGTATC119512700.089.1750647
CATGGGG20441950.025.21001225-29
ATGGGGT6008100.024.38554825-29
CGGTCCT4242550.023.76361760-64
GAGACGT4354600.023.69741640-44
ATGGGGG6027100.023.62504825-29
AAGACGC4405950.023.44870-74
TGGGTAA4725550.023.3931225-29
GTCCTAT4255350.023.28565460-64
ACCCCGG4396750.023.27011155-59
GGTCCTA4341400.023.21590660-64
CCGGTCC4318050.023.13565360-64
Produced by FastQC (version 0.11.5)