FastQCFastQC Report
Tue 21 Mar 2023
SRR9602588_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9602588_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105167743
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA71765736.823929843202968No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC31293042.9755359492691595No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA20314531.93163126073743No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT9419930.8957052544143692No Hit
AGATGTACTCTGCGTTGATACCCATGTACTCTGCGTTGATACCACTGCTT7683090.7305557560553524No Hit
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC5136070.4883693282264316No Hit
GCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA4203980.3997404413252455No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC3701730.35198340236321324No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC3386320.32199226715362717No Hit
CCTAATCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC2289340.21768461837200406No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG2173970.20671452462377177No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC1746850.16610131112160503No Hit
CATGTACTCTGCGTTGATACCCATGTACTCTGCGTTGATACCACTGCTTC1699070.16155809296011991No Hit
NGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA1533960.14585841211786774No Hit
AGATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTA1430710.13604076299326878Illumina Single End PCR Primer 1 (100% over 21bp)
CCATGTACTCTGCGTTGATACCCATGTACTCTGCGTTGATACCACTGCTT1382800.1314851836270747No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATGTA12427500.0143.703491
TGGAACA3690400.0140.60511
ATGTGAC5872300.0138.831045
GATGTAC12975600.0137.972952
GGAACAA3755900.0137.935032
TGACTGG4983950.0137.022728
GTGACTG4990700.0136.784167
ATAGCGA1543900.0136.682881
TATGTGA5448550.0135.662034
TGTGACT5090850.0134.825966
TAGCGAC1559250.0134.59622
CCGACAA1105350.0133.908431
GCCAAGA2136000.0133.584991
GTATGTG6849950.0132.386293
GACTGGA4184900.0132.358929
AACAGGC1835650.0132.292722
GATAGAC1124150.0132.093951
CCAAGAC2162300.0131.830612
AAGGACA1278050.0131.334871
GTGTATG7369350.0131.091581
Produced by FastQC (version 0.11.5)