FastQCFastQC Report
Tue 21 Mar 2023
SRR9602587_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9602587_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101726300
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA57541735.65652441895557No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA44447444.36931648944275No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC26912522.6455813295086914No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT17879501.7576084060857418No Hit
AGATGTACTCTGCGTTGATACCCATGTACTCTGCGTTGATACCACTGCTT6091120.5987753412834242No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG5842480.5743332845095123No Hit
ATCATTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC5497700.5404403777587506No Hit
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC5244830.5155824993143366No Hit
CCATGTACTCTGCGTTGATACCCATGTACTCTGCGTTGATACCACTGCTT4634060.45554197881963665No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC4467550.43917354705715234No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC2963470.29131797775010004No Hit
CCTAATCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC2731530.26851758099921064No Hit
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA2550840.25075521276208806No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC2034890.20003578229032215No Hit
CCATCCTCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG1655040.16269538949121318No Hit
CCATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTA1628910.16012673222165752Illumina Single End PCR Primer 1 (100% over 21bp)
CCCATGTACTCTGCGTTGATACCCATGTACTCTGCGTTGATACCACTGCT1602170.1574981101249136No Hit
ATCATTCCTATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC1468460.14435401661124017No Hit
AGATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTA1379780.13563650698000418Illumina Single End PCR Primer 1 (100% over 21bp)
GCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA1378200.13548118824728708No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC1349340.13264416380031516No Hit
CATGTACTCTGCGTTGATACCCATGTACTCTGCGTTGATACCACTGCTTC1342290.13195112768281161No Hit
NGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA1314780.12924681227961696No Hit
CCATGTACTCTGCGTTGATACCACCCATGTACTCTGCGTTGATACCACTG1139610.11202707657705038No Hit
CCATGTACTCTGCGTTGATACCCCATGTACTCTGCGTTGATACCACTGCT1036930.10193332501034638No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATGTA9865700.0142.427381
GATAGAC2191450.0140.229931
ATAGCGA2062450.0139.695251
CCGACAA2111600.0138.670641
TAGCGAC2105900.0137.670932
GATGTAC10457450.0135.160952
ATAGACA2279500.0134.904632
TCACGCA1915500.0133.571852
AGAGATC2568050.0132.733572
TGGAACA2094100.0132.186131
CGACAAC2229500.0132.102772
AAGGACA1883450.0131.532731
AGGACAC1899750.0131.36222
GGCTAAC1019850.0130.130922
TTCACGC1981550.0128.93531
GGAACAA2156450.0128.796422
AACAGGC1616900.0128.45492
AGTTAGC947850.0127.29682
AGGCTAA1037800.0126.70941
ACCTTAC1582100.0126.44162
Produced by FastQC (version 0.11.5)