FastQCFastQC Report
Tue 21 Mar 2023
SRR9602586_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9602586_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77218368
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC56260937.285951705169423No Hit
CAACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA12165351.5754476965894955No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10617181.3749552438093484No Hit
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT6401190.8289724538078815No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC5376040.6962125902479576No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT1833610.2374577509848434No Hit
CATGTACTCTGCGTTGATACCACTGCTTAGATCGGAAGAGCGTCGTGTAG1819140.23558384450704786Illumina Single End PCR Primer 1 (100% over 22bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA1302290.1686502879729341No Hit
CATGTACTCTGCGTTGATACCCATGTACTCTGCGTTGATACCACTGCTTC1258840.16302338842488875No Hit
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC1243260.16100573376531346No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA1159490.15015727864126835No Hit
CATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT1136820.14722144865843317No Hit
NATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC1051820.13621370500863214No Hit
CAACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC904350.11711591729055967No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGGA4325550.0136.240631
ATGCCTA1190650.0133.472381
AACGTGA1746750.0132.871481
CAACCAC5049900.0132.228321
CTCAATG1332200.0131.87881
ACGTGAT1748100.0131.785262
ACGCTTA1125400.0131.74942
TGCCTAA1183250.0130.985932
CGCATAC1022250.0127.692031
TCAATGA1397050.0125.609282
AATGGAA4803750.0123.0248262
AACATCG865300.0122.054192
AACCACA2767600.0121.1452562
CAAGCTA716900.0120.943382
GCCTAAT473850.0120.142273
AACGCTT1264400.0117.737381
GCATACA1097100.0117.564452
GATCGCA967250.0116.581912
ACAAGCT746800.0116.5071
AACCACT2809350.0115.876992
Produced by FastQC (version 0.11.5)