FastQCFastQC Report
Tue 21 Mar 2023
SRR9602585_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9602585_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28326402
Sequences flagged as poor quality0
Sequence length150
%GC28

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC500340.17663379909668725No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAC1719100.0145.083761
CGACTGG1412200.0143.106951
ACAAGCT1596250.0142.04431
GAGCTGA1013400.0141.416661
CAAGCTA1607600.0141.114062
AAGACGG1302050.0141.00061
GCATACA1766800.0140.934652
ACGCTTA1027600.0140.265752
AGTCACT864000.0140.177661
AGAGTCA858400.0140.117261
ATTGAGG3507600.0140.09321
AGATCGC389700.0139.956571
CGAACTT696450.0139.793431
GTCACTA867650.0139.45982
AGACGGA1317250.0138.945762
GACTAGT448150.0138.918011
ACTTCGA654950.0138.87342
CAATGGA1645400.0138.225081
AGCTGAA1037650.0137.977372
GACGGAT484550.0137.843293
Produced by FastQC (version 0.11.5)