FastQCFastQC Report
Tue 21 Mar 2023
SRR9602585_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9602585_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28326402
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG5786892.04293153786351No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1895510.6691672313342161No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA603570.21307683199581787No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT13711300.0113.977211
AGTGGTA13918300.0112.3233645
GTGGTAT13940400.0112.137366
TGGTATC13966800.0111.954347
GGTATCA13984100.0111.887178
AGCAGTG13981200.0111.6883852
CAGTGGT14033300.0111.410084
GTATCAA14051350.0111.378329
GCAGTGG14192900.0110.008213
CATGGGG3483600.025.99499925-29
ATGGGAT2075700.025.37139725-29
ATGGGGT974950.024.5477325-29
ATGGGAG1728150.024.39975425-29
ATGGGAC1321850.023.89308225-29
ATGGGGG1160900.023.45736125-29
TACATGG13284300.023.07188620-24
AGTACAT13651750.023.00789520-24
GTACATG13424600.022.9827520-24
ACATGGG13263550.022.97054120-24
AGAGTAC13811350.022.9455715-19
Produced by FastQC (version 0.11.5)