FastQCFastQC Report
Tue 21 Mar 2023
SRR9602584_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9602584_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33403684
Sequences flagged as poor quality0
Sequence length150
%GC27

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAGTA3166350.0146.030081
GCTAACG3462300.0145.994661
GATAGAC2987250.0145.826631
TATCAGC2509400.0144.838521
GCCACAT3834300.0144.747591
GGAGAAC5280600.0144.480851
GTCGTAG1178600.0143.840481
CTAACGA3532200.0143.063722
GGTGCGA2227600.0142.997621
GTGCGAA2240850.0141.868712
ATCAGCA2572300.0141.6712
GAGAACA5385000.0141.610752
TCGTAGA1196650.0141.400122
CGAGTAA3274150.0141.164222
CCACATA3933750.0141.142782
ATAGACA3086600.0141.017232
TAACGAG1224800.0140.929583
TAACGAT1320250.0140.91653
TGCGAAT856800.0139.479753
GTACGCA252600.0139.409321
Produced by FastQC (version 0.11.5)