FastQCFastQC Report
Tue 21 Mar 2023
SRR9602584_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9602584_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33403684
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1363170.40808971848733816No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG421410.12615674366935095No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG336110.10062063813081215No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT12635350.0138.094421
GTGGTAT12655600.0137.919116
GGTATCA12676800.0137.809138
TGGTATC12706700.0137.392857
GTATCAA12736600.0137.197719
AGTGGTA12731650.0137.092095
AGCAGTG12831500.0135.894152
CAGTGGT12902350.0135.286714
GCAGTGG12954400.0134.580153
CAACGCA12604350.028.2281610-14
TCAACGC12607700.028.19404610-14
AACGCAG12696350.028.01771710-14
ATCAACG12685100.027.995810-14
CGCAGAG12673700.027.99055315-19
TACATGG12290200.027.91622520-24
GTACATG12372700.027.90593120-24
AGAGTAC12703300.027.84360715-19
CAGAGTA12729250.027.7956715-19
AGTACAT12657150.027.74612620-24
GCAGAGT12741150.027.7330915-19
Produced by FastQC (version 0.11.5)