FastQCFastQC Report
Tue 21 Mar 2023
SRR9602583_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9602583_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31992811
Sequences flagged as poor quality0
Sequence length150
%GC28

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA709250.2216904291404716No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA525320.16419938841885445No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAGTA1645800.0144.80761
GATAGAC1116500.0144.417831
TATCAGC1036400.0142.053541
AGAGATC2220500.0140.897862
CGAGTAA1695650.0140.512822
TCACGCA1312200.0139.787892
ATCCGTC811350.0139.472672
GTCTGTC2001700.0139.45781
ATCAGCA1060900.0139.249862
GTGCGAA920350.0139.09442
AATCCGT817550.0138.834581
GAGATCA675150.0137.57253
ATAGACA1174600.0137.338072
TTCACGC1330200.0137.253481
GCCACAT830500.0137.244461
ATAGCGA214750.0137.050721
TGGAACA1218600.0136.933721
GGTGCGA937100.0136.849931
GAGATCT836100.0136.827743
CCGACAA478850.0136.69731
Produced by FastQC (version 0.11.5)