FastQCFastQC Report
Tue 21 Mar 2023
SRR9602583_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9602583_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31992811
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG2320920.7254504769837199No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1570560.49091028606395354No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA546550.17083525420757809No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC469460.146739215881968No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCGCGGTGGCTCA379830.11872354698685275No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT11171400.0124.860351
GTGGTAT11237500.0124.171196
GGTATCA11262000.0124.001738
TGGTATC11267050.0123.8665547
AGTGGTA11298200.0123.504275
GTATCAA11319400.0123.4079069
AGCAGTG11375050.0122.542592
CAGTGGT11432400.0122.070884
GCAGTGG11764650.0118.4859853
CATGGGG4042850.026.11484525-29
CAACGCA11218500.025.36861210-14
TCAACGC11232900.025.31122210-14
ACATGGG10789450.025.29443420-24
TACATGG10914300.025.20337520-24
CGCAGAG11282900.025.15204615-19
ATCAACG11299650.025.14258810-14
GTACATG11012050.025.13224820-24
AACGCAG11334100.025.11838210-14
AGAGTAC11289950.025.08532515-19
CAGAGTA11308550.025.03795215-19
Produced by FastQC (version 0.11.5)