FastQCFastQC Report
Tue 21 Mar 2023
SRR9602582_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9602582_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26611120
Sequences flagged as poor quality0
Sequence length150
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC1151680.43278148383081955No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAGAC1844900.0144.976641
AACGTGA1336700.0143.867921
AGATCGC1804250.0143.727071
ACGTATC627100.0142.828631
CGCATAC833300.0142.673521
ATGCCTA679250.0142.533751
AACATCG2142700.0142.059622
GATCGCA1823400.0141.852222
ATAGACA1898650.0141.475592
AAACATC2165700.0141.465031
ACGTGAT1350500.0140.973562
ACAGCAG1312650.0140.92631
CGAACTT776200.0140.542421
AACCGAG606100.0140.102451
CAAGCTA780700.0139.975132
ACATCGA656450.0139.671133
ATCGCAT656300.0139.648213
CAGCAGA1326900.0139.239972
GCATACA853800.0139.14982
ACTTCGA644300.0138.816072
Produced by FastQC (version 0.11.5)