FastQCFastQC Report
Tue 21 Mar 2023
SRR9602582_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9602582_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26611120
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG2896531.0884660247295115No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1231510.4627802212007611No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAAAGCAAGCAGGAGACGTG749090.28149510430226166No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG630590.2369648477779214No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA533510.2004838578759556No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC310030.11650392768136027No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT8248950.0114.289971
GTGGTAT8328950.0112.8452156
TGGTATC8331450.0112.839017
GGTATCA8342650.0112.782878
GTATCAA8373800.0112.392189
AGTGGTA8399250.0111.922155
AGCAGTG8433650.0111.7780462
CAGTGGT8488400.0111.0481344
GCAGTGG8683700.0108.539833
CATGGGG2000150.024.93025825-29
CAACGCA8305400.023.16462510-14
ACATGGG7878700.023.13361720-24
AGAGTAC8283900.023.07050715-19
ATCAACG8310750.023.04507310-14
TACATGG8036900.023.04479420-24
GTACATG8099950.023.02219620-24
AGTACAT8232850.023.01974320-24
TCAACGC8353900.023.01100710-14
CAGAGTA8329500.022.95993815-19
CGCAGAG8360750.022.94266115-19
Produced by FastQC (version 0.11.5)