FastQCFastQC Report
Tue 21 Mar 2023
SRR9602581_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9602581_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38387327
Sequences flagged as poor quality0
Sequence length150
%GC30

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC1988130.5179131123143844No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAGAC1704450.0144.169141
CGAACTT2475950.0143.661061
AACCGAG2108250.0143.452411
CGCATAC1338650.0143.194611
CGACTGG2037200.0142.748171
AACGTGA1514600.0142.731521
GAGCTGA1804200.0142.167051
GACTAGT1000600.0141.866741
ACCGAGA2123950.0141.604112
ACGTATC596350.0141.091191
ACAAGCT2231500.0140.950041
AGTCACT1452450.0140.763171
CCGTGAG1348800.0140.659481
ACGTGAT1527900.0140.632132
CAAGCTA2236600.0140.45992
GAACTTA2525650.0140.321262
GTCACTA1455950.0139.942022
CGTGAGA1361800.0139.200732
ACGCTTA924350.0138.66692
AGCTGAA1846300.0138.624732
Produced by FastQC (version 0.11.5)