FastQCFastQC Report
Tue 21 Mar 2023
SRR9602581_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9602581_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38387327
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG11129222.8991911835903554No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA2089270.5442603492553676No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG2067550.5386022319293031No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1926980.5019833759198706No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAAAGCAAGCAGGAGACGTG1015310.26449093472957885No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC923590.24059763265100484No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC609500.15877635866649428No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA606270.15793493514148563No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT23349550.0110.1829151
GTGGTAT23732350.0108.340136
TGGTATC23742450.0108.303147
AGCAGTG23781750.0108.143972
AGTGGTA23817900.0107.960465
GGTATCA23837250.0107.940678
GTATCAA23878100.0107.7813349
CAGTGGT23858750.0107.7713854
GCAGTGG24214650.0106.20473
CATGGGG3946850.025.2379525-29
ATGGGAT3143200.025.10691625-29
ATGGGAG3309800.024.67657925-29
ATGGGAC1581200.024.06308725-29
CATGGGT2123700.023.9212125-29
AAACCGC438950.022.77695585-89
ACATGGG21585350.022.45670120-24
GAAACCG447350.022.44262785-89
CGCAGAT451450.022.2643890-94
AGTACAT23241900.022.17188620-24
AGAGTAC23500050.022.15089815-19
Produced by FastQC (version 0.11.5)