FastQCFastQC Report
Tue 21 Mar 2023
SRR9602580_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9602580_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24899898
Sequences flagged as poor quality0
Sequence length150
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC381400.15317331822001842No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAGT1387200.0143.77211
AAGGTAC1506800.0142.994451
AGATCGC1023000.0142.462521
ACGCTTA1756700.0142.207932
AGAGTCA875050.0141.827871
AACGTGA680150.0141.416371
ACGTATC439900.0141.394641
GATAGAC478150.0141.310151
AACCGAG691700.0141.137861
GATCGCA1034100.0140.687292
AGGTACA1535400.0140.560462
ACTAGTA1426700.0140.370352
GAGTCAA885850.0139.797672
GAGCTGA693200.0139.492611
ACCGAGA700100.0139.352512
ACGTGAT687900.0139.020522
CGTGAGA894300.0138.782522
ACACAGA1242700.0138.674091
CGCATAC385300.0138.635471
CAAGCTA530450.0138.598392
Produced by FastQC (version 0.11.5)