FastQCFastQC Report
Tue 21 Mar 2023
SRR9602580_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9602580_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24899898
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG1635470.6568179516237376No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1445800.5806449488266979No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAAAGCAAGCAGGAGACGTG894400.3591982585631475No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG747860.3003466118616229No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC396000.1590367960543453No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC376520.1512134708342982No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA291730.11716112250740947No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT6975500.0124.081311
GGTATCA6989800.0123.54458
GTGGTAT6982100.0123.534626
TGGTATC6990200.0123.407957
GTATCAA7038600.0122.730039
AGTGGTA7051600.0122.316055
AGCAGTG7099900.0121.872172
CAGTGGT7139300.0121.180324
GCAGTGG7265850.0119.075663
CAACGCA6975300.025.27966910-14
TCAACGC6996750.025.174810-14
ATCAACG6983100.025.1293710-14
GTACATG6719500.025.08026920-24
AGAGTAC6979050.025.06830415-19
TACATGG6635350.025.01209620-24
CGCAGAG7028500.025.00173415-19
AGTACAT6928500.024.98579820-24
CAGAGTA7014550.024.96647815-19
AACGCAG7092300.024.87197710-14
GCAGAGT7057800.024.84816615-19
Produced by FastQC (version 0.11.5)