FastQCFastQC Report
Tue 21 Mar 2023
SRR9602579_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9602579_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26427974
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC5957952.254410421320984No Hit
CTGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC1323480.5007875367215058No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT766690.29010547687083393No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC674940.2553884758627355No Hit
ACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT552250.20896418317953547No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAGAC1066000.0143.963551
CGCATAC1454250.0143.947221
GCCACAT1072100.0141.525091
ATTGAGG2696750.0141.38851
GCATACA1479750.0141.321432
CGAACTT692200.0141.08781
ACGTATC451800.0140.928631
ATAGACA1099100.0140.086442
GACTAGT580150.0139.510991
AGATCGC825250.0139.440431
AACGTGA446550.0139.353871
GAACTTA700800.0139.199662
AGCAGGA919400.0138.992771
TTGAGGA2742200.0138.779682
AAGGTAC559050.0138.631121
ACTAGTA588000.0138.562762
ACAGCAG944450.0137.920041
AACATCG460400.0137.311582
GATCGCA832550.0137.189872
AGGTACA566750.0136.838122
Produced by FastQC (version 0.11.5)