FastQCFastQC Report
Tue 21 Mar 2023
SRR9602579_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9602579_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26427974
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG332406012.577808650787986No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA6206272.3483714642673705No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1305060.4938176494346483No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCAGACGTGTGCTCTTCCGA1002840.3794615508551658TruSeq Adapter, Index 2 (95% over 22bp)
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA889780.3366811243268213No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA665790.2519262354352248No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT544810.20614898440569074No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG530950.20090454152860904No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAAAGCAAGCAGGAGACGTG479910.18159167252094316No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG479110.18128896297536845No Hit
AAGCAGTGGTATCAACGCAGAGTACATAGGAAGCAGTGGTATCAACGCAG474280.1794613540939612No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT460790.1743569143817078No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA424130.16048524945574716No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT421610.15953171438718686No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA395950.14982230571287833No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC388480.14699575533107456No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG384810.14560707529075065No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG379150.1434654052558096No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC369490.13981018749299512No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA360010.13622307937793493No Hit
NAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG357790.13538306038896514No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG349530.13225758433090634No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG341270.12913210827284755No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA325000.1229757528897221No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA288800.10927814595246689No Hit
AGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT286150.10827542058275069No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAG273820.10360990971158063No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT26244800.046.2204361
AGCAGTG26863050.045.1916852
AGTGGTA26899950.044.9867485
CAGTGGT27043650.044.8764
GCAGTGG27377750.044.3338853
GTATCAA27340800.044.2900479
GTGGTAT27363650.044.2209856
TGGTATC27401100.044.161347
GGTATCA27497750.044.0263528
GTGACTG160750.026.376351
TGACTGG185000.022.8858492
AGTTCAG189450.022.4580129
GACTGGA209000.020.4278663
CGCATAC32500.019.4351861
CATGGGG1547700.019.35576225-29
TGGAGTT220550.018.93276
CGATAGG27900.017.6496935-39
ACTGGAG244550.017.1054
AAACCGC142050.016.97786185-89
ATGGGGG495750.016.95547125-29
Produced by FastQC (version 0.11.5)