FastQCFastQC Report
Tue 21 Mar 2023
SRR9602578_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9602578_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78322547
Sequences flagged as poor quality0
Sequence length150
%GC29

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC1736400.22169861253362966No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACT5726050.0143.634461
GATAGAC2379700.0143.525381
GACTAGT2690250.0143.120991
GAGCTGA3751650.0143.044971
CGCATAC1404050.0142.391651
GTCACTA5725250.0142.300612
ACGCTTA3463700.0141.849272
CCGTGAG2719100.0141.61591
CGACTGG2356950.0141.526731
ACGTATC699500.0141.165511
CGAACTT2202250.0140.755981
CGTGAGA2679000.0140.540372
AACATCG2420550.0140.188922
ACCGAGA2423600.0140.021412
AACCGAG2477400.0139.954511
CGCTGAT1088100.0139.696291
CGCTTAG1169200.0139.561523
TCACTAG1932050.0139.560843
AGCTGAA3812450.0139.128922
CCGAAGT1461000.0138.752691
Produced by FastQC (version 0.11.5)