FastQCFastQC Report
Tue 21 Mar 2023
SRR9602578_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9602578_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78322547
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG4520460.5771594736315202No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG1996410.2548959496937708No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1673830.2137098529239607No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC1050570.1341337890863023No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA793600.10132459047839698No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT19778500.0124.9409261
GGTATCA19912000.0124.061518
GTGGTAT19942400.0123.801636
TGGTATC19963950.0123.6705257
GTATCAA20001100.0123.529369
AGTGGTA20078250.0122.961675
AGCAGTG20296900.0121.70462
CAGTGGT20487500.0120.5486454
GCAGTGG20992800.0117.647513
CAACGCA19715000.025.4740110-14
CATGGGG7215050.025.3479725-29
TCAACGC19827500.025.30681610-14
ATCAACG19826050.025.27126910-14
AGAGTAC19796000.025.26570315-19
GTACATG19392000.025.18915220-24
CAGAGTA19908300.025.12607615-19
AGTACAT19737050.025.11191720-24
CGCAGAG19913150.025.10072515-19
TACATGG19343750.025.09582720-24
GAGTACA19674700.024.96581620-24
Produced by FastQC (version 0.11.5)