FastQCFastQC Report
Tue 21 Mar 2023
SRR9602577_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9602577_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94011772
Sequences flagged as poor quality0
Sequence length150
%GC29

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC1372450.14598703660218212No Hit
CTGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC958430.10194787095386311No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAC2730200.0143.636551
ACGTATC1792900.0143.552411
AACCGAG6241500.0142.805311
CGAACTT4262600.0142.56491
GACTAGT2309150.0142.33851
ACGCTTA5654750.0142.273332
CCGTGAG4022800.0142.170531
GATAGAC1974950.0142.154951
ACCGAGA6131150.0141.863342
CGTGAGA3950500.0141.071722
GAGCTGA2560300.0140.872011
AACATCG3605950.0140.705232
AACGTGA2323850.0140.572921
CGCTTAG1938900.0140.314973
GACGGAT1741350.0140.234563
ACGTGAT2336300.0140.219792
CCGAGAT2321100.0139.90223
AGAGTCA2683900.0139.603871
AAGACGG4907350.0139.250951
ATGCCTA1500350.0138.465621
Produced by FastQC (version 0.11.5)