FastQCFastQC Report
Tue 21 Mar 2023
SRR9602577_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9602577_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94011772
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG4393400.4673244537928718No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG2271640.24163356903856678No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1724620.18344723892663145No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA1282050.13637121955322787No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC1248810.1328354921339No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA954110.10148835403293961No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT22815100.0125.4406051
GGTATCA22869600.0125.147298
GTGGTAT22921300.0124.7783366
TGGTATC22948100.0124.649257
GTATCAA22993700.0124.478459
AGTGGTA23066950.0123.9838265
AGCAGTG23427800.0122.133672
CAGTGGT23678550.0120.8300554
GCAGTGG24125400.0118.5936663
CAACGCA22597000.025.74318510-14
TCAACGC22720250.025.5785710-14
ATCAACG22791000.025.4651810-14
AGAGTAC22774500.025.42167515-19
GTACATG22197300.025.40483720-24
TACATGG22064000.025.34813920-24
AGTACAT22647300.025.32306120-24
AACGCAG22983350.025.29778710-14
CGCAGAG22889250.025.29360215-19
CAGAGTA22909850.025.2786515-19
ACATGGG21833150.025.24627720-24
Produced by FastQC (version 0.11.5)