FastQCFastQC Report
Tue 21 Mar 2023
SRR9602576_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9602576_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99027818
Sequences flagged as poor quality0
Sequence length150
%GC28

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTGA5947900.0143.742421
GTCGTAG2830200.0142.778811
ATGCCTA3362000.0142.639681
TATCAGC3402850.0142.380421
ATAGCGA1269250.0142.282181
ACGTGAT6032600.0141.922562
AGTGGTC6347500.0141.778931
ATCCGTC3518850.0141.443652
TCACGCA5632250.0141.259572
TAGCGAC1274050.0141.07822
CGACACA2776700.0140.392721
GTGGTCA6413050.0140.232222
TGAAGAG6948500.0140.052411
CACGCAT2707800.0139.8963
GTACGCA726300.0139.718861
AATCCGT3580200.0139.601271
TTCACGC5720950.0139.576081
ATCAGCA3465250.0139.542772
TCGTAGA2902550.0139.510312
AGCACCT3981400.0139.336321
Produced by FastQC (version 0.11.5)