FastQCFastQC Report
Tue 21 Mar 2023
SRR9602576_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9602576_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99027818
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG2543070.25680359835859456No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG2342580.23655777207976045No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTGCGATAGGAATCATGTC1920730.19395863089702733No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1668940.16853244206592535No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA1215120.12270491509769509No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC1177200.11887568804151577No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACTGCGATAGGAATCATGT1173560.11850811455827494No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT32624700.0135.196721
GTGGTAT32735650.0134.657876
GGTATCA32778850.0134.569618
TGGTATC32809250.0134.358147
GTATCAA32951000.0133.9079
AGTGGTA33033350.0133.466985
AGCAGTG33271150.0132.52932
CAGTGGT33452300.0131.808694
GCAGTGG34075650.0129.376593
CAACGCA32501250.027.53336510-14
TCAACGC32632000.027.40080310-14
ATCAACG32749150.027.27451710-14
CGCAGAG32795150.027.20650115-19
GTACATG31679900.027.20437420-24
AGAGTAC32824300.027.188715-19
TACATGG31171750.027.18138720-24
AACGCAG32931350.027.17625610-14
CAGAGTA32916400.027.1242615-19
AGTACAT32717900.027.03595520-24
GCAGAGT33091450.026.95967515-19