FastQCFastQC Report
Tue 21 Mar 2023
SRR9602575_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9602575_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88683945
Sequences flagged as poor quality0
Sequence length150
%GC29

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA2110040.23792807142262334No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAGC3815450.0142.68021
ATAGCGA1489050.0142.007231
ATCCGTC4267300.0141.977522
TAGCGAC1491150.0140.903562
GAGTTAG4707500.0140.878311
AGTTAGC4702250.0140.75162
TCACGCA4365950.0140.044952
ATCAGCA3892300.0139.842942
AGCACCT3885450.0138.95051
CACGCAT2084000.0138.665593
AATCCGT4403900.0138.578951
GGCTAAC3565900.0138.56242
TTCACGC4425950.0138.375961
AGCCATG2027950.0138.18911
GCTAACT1740400.0138.172013
AGGCTAA3603200.0137.923451
GTTAGCG1338150.0137.617453
CGGATTG2078900.0137.431661
CAGATTC4016700.0137.402252
AACATCG1837950.0137.363452
Produced by FastQC (version 0.11.5)