FastQCFastQC Report
Tue 21 Mar 2023
SRR9602575_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9602575_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88683945
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG3541920.39938683377244893No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG2631290.29670421179391604No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTGCGATAGGAATCATGTC2195540.24756904984323827No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA1504390.16963498861039616No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACTGCGATAGGAATCATGT1401820.1580691973051041No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC1250070.1409578701082817No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT1193640.13459482435067588No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA1127830.12717408996634058No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC1004350.11325048744730513No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT35349100.0134.13381
GGTATCA35643100.0133.044348
GTGGTAT35667650.0132.893236
TGGTATC35721200.0132.694237
GTATCAA35854400.0132.286979
AGTGGTA35861600.0132.188375
AGCAGTG36094550.0131.341462
CAGTGGT36261800.0130.747154
GCAGTGG36971350.0128.2083
CAACGCA35382850.027.16748610-14
TCAACGC35615650.026.9693310-14
TACATGG34446650.026.94133620-24
GTACATG34725550.026.92645820-24
ATCAACG35643200.026.92142110-14
CGCAGAG35665400.026.87110115-19
AACGCAG35772050.026.87045310-14
AGAGTAC35712950.026.86184915-19
ACATGGG34256000.026.8156520-24
CAGAGTA35797900.026.8048215-19
AGTACAT35566300.026.77709220-24
Produced by FastQC (version 0.11.5)