FastQCFastQC Report
Tue 21 Mar 2023
SRR9602574_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9602574_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97975788
Sequences flagged as poor quality0
Sequence length150
%GC30

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGA2841700.0143.582291
AACGTGA4910100.0142.694151
TAGCGAC2856750.0142.207642
AACATCG7025650.0141.806442
ACACGAC4618300.0141.322481
ACGTGAT4979250.0141.02092
TCACGCA5607200.0140.760072
ACATCGA2383300.0140.481463
ATCCGTC2984700.0140.383672
TGGAACA8729650.0140.341461
CATCGAT1262950.0139.381124
CACGCAT2673650.0139.350563
ACATCGT3615600.0139.252063
TTCACGC5688300.0139.077841
ACGACCT2293500.0138.750243
TATCAGC1840050.0138.66151
GTGCGAA2170350.0138.487412
AGCGACT1367400.0138.433793
CACGACC4699750.0138.059632
AGAGATC2528550.0137.194662
Produced by FastQC (version 0.11.5)