FastQCFastQC Report
Tue 21 Mar 2023
SRR9602574_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9602574_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97975788
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG5799770.5919595155488824No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG4526070.4619580094625011No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA2276240.2323267866955048No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA2170870.22157208881034973No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC2074870.21177374965333273No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT1578020.16106224121412527No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1176010.12003067533378757No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG1014490.1035449697021064No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCCTTTCCGGCGGTGACGACC1002170.10228751617695588No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT38454550.0130.282231
GTGGTAT38984550.0128.505866
GGTATCA39019500.0128.449438
TGGTATC39058450.0128.261617
GTATCAA39166550.0128.00749
AGTGGTA39297600.0127.487295
AGCAGTG39307400.0127.450292
CAGTGGT39568800.0126.634774
GCAGTGG40327550.0124.223953
CAACGCA38746550.026.23370210-14
CATGGGG14541400.026.20039425-29
ACATGGG36921750.026.11241520-24
GTACATG37857050.026.09594220-24
TACATGG37544150.026.09277320-24
AGAGTAC38920450.026.0653115-19
CAGAGTA39000050.026.01838515-19
TCAACGC39053900.026.0092910-14
ATCAACG39024500.025.9994110-14
AGTACAT38710600.025.99515720-24
GCAGAGT39100500.025.91652515-19
Produced by FastQC (version 0.11.5)