FastQCFastQC Report
Tue 21 Mar 2023
SRR9602573_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9602573_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86405533
Sequences flagged as poor quality0
Sequence length150
%GC30

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA1437310.16634467146912918No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGA3485550.0143.111711
TATCAGC5085500.0142.169141
TAGCGAC3513900.0141.397372
ATCAGCA5158150.0140.062582
AGCACCT4306450.0139.167051
AGCGACT1665650.0138.397083
TCACGCA3436500.0138.293432
AACATCG2744300.0138.113332
AGAGATC3440500.0137.910692
ATCCGTC1521950.0137.220522
CACGCAT1650600.0137.036323
AGTTAGC2331250.0136.929282
AGGACAC4665350.0136.860932
ACACGAC1730750.0136.331021
AAGGACA4709000.0136.114031
GAGTTAG2351750.0135.858441
GCACCTC4394000.0135.814072
TCAGCAT2817700.0135.474963
ACATCGA950550.0135.264923
TAGGATG2468750.0134.992031
Produced by FastQC (version 0.11.5)