FastQCFastQC Report
Tue 21 Mar 2023
SRR9602573_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9602573_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86405533
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG8294210.9599165368264091No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG3259000.37717492003666014No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA3112160.36018063796909855No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA1819470.21057332057658856No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC1264550.14635058150732083No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1238950.14338780827843514No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA1219950.1411888750226215No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT1200700.13896100843449458No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT36924700.0124.833221
GGTATCA37518200.0122.7631768
GTATCAA37607950.0122.589079
GTGGTAT37558150.0122.579886
TGGTATC37619100.0122.382817
AGCAGTG37704000.0122.1204762
AGTGGTA37771750.0121.9055565
CAGTGGT37942100.0121.3629764
GCAGTGG38576650.0119.350513
CATGGGG9565650.025.77067825-29
ACATGGG34318500.025.20339220-24
CAACGCA37281050.025.05013810-14
AGAGTAC37257350.024.9797815-19
CAGAGTA37343850.024.94289415-19
ATCAACG37451400.024.91163310-14
GTACATG36129000.024.9021320-24
AGTACAT37076400.024.90135420-24
TACATGG35606900.024.89966420-24
GCAGAGT37463200.024.82749715-19
CGCAGAG37499850.024.8157515-19
Produced by FastQC (version 0.11.5)