FastQCFastQC Report
Tue 21 Mar 2023
SRR9602572_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9602572_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84376725
Sequences flagged as poor quality0
Sequence length150
%GC29

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA2424760.287373087779835No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC1603810.19007729916040236No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGA1333800.0142.623691
AACGTGA3458100.0141.663911
CGACACA3904600.0141.64021
GAGTTAG4755950.0141.389251
TAGCGAC1343450.0141.136552
AGTTAGC4757850.0141.102862
AGCACCT4619750.0140.59461
ACGTGAT3503050.0140.204792
AACATCG2894200.0139.913332
AGAGATC3290000.0139.54912
CGGATTG2753700.0139.548031
GTTAGCG1325100.0138.893463
ATGCCTA1350550.0138.626831
GAACAGG7226450.0138.581331
GCACCTC4689050.0138.244772
TATCAGC1482550.0138.06871
GTTAGCT2066200.0137.689363
AACAGGC7280050.0137.47492
GTACGCA490300.0137.36421
ACATCGA1000000.0137.213763
Produced by FastQC (version 0.11.5)