FastQCFastQC Report
Tue 21 Mar 2023
SRR9602572_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9602572_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84376725
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG4290160.5084530123680434No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG2998040.35531599502113886No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA2283780.2706646886330324No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC1445100.1712676096399807No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1134300.13443280715149825No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTGCGATAGGAATCATGTC1006900.11933385658189506No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA849110.10063320186935437No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT23403000.0123.758851
GGTATCA23521550.0123.165278
TGGTATC23577550.0122.815327
GTGGTAT23590550.0122.7430656
GTATCAA23660100.0122.472569
AGTGGTA23806300.0121.6391455
AGCAGTG23968100.0120.827972
CAGTGGT24131200.0120.0245364
GCAGTGG24529050.0118.051193
ACATGGG21829650.025.28836620-24
CATGGGG8711100.025.23515125-29
CAACGCA23294800.025.22396510-14
TCAACGC23473550.025.01268610-14
GTACATG22820750.024.97279720-24
ATCAACG23492250.024.96936610-14
AGAGTAC23513700.024.94197815-19
TACATGG22665050.024.91903120-24
CGCAGAG23563850.024.85974915-19
AGTACAT23412950.024.84127820-24
CAGAGTA23622850.024.83282915-19
Produced by FastQC (version 0.11.5)