FastQCFastQC Report
Tue 21 Mar 2023
SRR9602571_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9602571_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95137476
Sequences flagged as poor quality0
Sequence length150
%GC31

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA1548400.16275394987354933No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGCGA7586800.0142.247161
GTGCGAA7598700.0142.164782
ATAGCGA1847550.0142.024871
TAGCGAC1854950.0140.950582
TGCGAAT3801900.0140.655183
TCACGCA4966400.0140.338362
GTCGTAG1682300.0140.051651
AACATCG3904700.0140.005282
TATCAGC2372100.0139.784181
ACGTGAT2955050.0139.422032
AACGTGA2948100.0139.30141
TAGGATG3802100.0139.104861
ATGCCTA1838300.0139.053711
TTCACGC5035200.0138.815831
TGAAGAG6433800.0138.479321
GGCTAAC4078200.0138.30092
GCTAACT1994750.0138.285233
CACGCAT2364300.0138.246773
CCGTCTA2586400.0138.195362
GGCTTCA7142700.0137.295032
Produced by FastQC (version 0.11.5)