FastQCFastQC Report
Tue 21 Mar 2023
SRR9602571_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9602571_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95137476
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG6952390.7307730131499389No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG4358550.45813176712823456No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA2799820.29429202010782796No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA2172170.22831906955362155No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC1891930.1988627488919298No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT1595950.16775198030269375No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACTCGGTGGTGGCCACTGC1264670.1329307916472369No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1025230.10776300182695618No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTGCGATAGGAATCATGTC987400.10378665080414788No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT37025700.0127.066791
GGTATCA37538450.0125.3392948
GTGGTAT37612700.0125.0375066
TGGTATC37646900.0124.927357
AGTGGTA37699850.0124.754385
GTATCAA37724450.0124.749369
AGCAGTG37915200.0124.060892
CAGTGGT38148850.0123.315164
GCAGTGG38690700.0121.5602653
CATGGGG13271550.025.7956725-29
ACATGGG35174650.025.60365120-24
CAACGCA37317150.025.59014910-14
AGAGTAC37387100.025.47728515-19
CAGAGTA37459000.025.43438115-19
GTACATG36432000.025.43365320-24
TACATGG36115800.025.42108320-24
ATCAACG37547000.025.38356610-14
AGTACAT37224700.025.38189720-24
TCAACGC37594250.025.38145810-14
AACGCAG37817100.025.2550410-14
Produced by FastQC (version 0.11.5)