FastQCFastQC Report
Tue 21 Mar 2023
SRR9602570_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9602570_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95702710
Sequences flagged as poor quality0
Sequence length150
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA22720502.37407070290904No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA9011310.9415940259162986No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC3922690.4098828549369187No Hit
AGATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC1889770.19746253789469492No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1478600.15449928220423434No Hit
CCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG1375230.1436981251628089No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGA2507350.0143.64781
AACGTGA4799150.0143.157321
TAGCGAC2517400.0142.03292
ACGTGAT4874800.0141.279342
TATCAGC2963300.0140.974441
GAGTTAG4148100.0140.499981
AGTTAGC4124700.0140.082822
AACATCG3009750.0139.61562
TCACGCA3050850.0139.401032
AGTGGTC3896050.0139.001341
GATGTAC4159300.0138.915772
GTGCGAA1891050.0138.110762
GTCGTAG1148250.0138.052891
GTGGTCA3925750.0137.740862
TAGGATG3137700.0137.714431
AGCGACT1189900.0137.688063
ATCAGCA3033350.0137.435872
ATGCCTA1280300.0137.179381
CACGCAT1474450.0137.108473
TTCACGC3123750.0136.610291
Produced by FastQC (version 0.11.5)