FastQCFastQC Report
Tue 21 Mar 2023
SRR9602570_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9602570_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95702710
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG80840128.447004269785046No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA11929561.2465226951253523No Hit
AAGCAGTGGTATCAACGCAGAGTACATAGGAAGCAGTGGTATCAACGCAG1577860.16487098432217856No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA1456320.1521712394560196No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG1058810.11063532056720232No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG1015800.10614119495675724No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT68261250.061.9462241
AGTGGTA70508350.059.9920925
AGCAGTG71322750.059.2952962
GTATCAA71753850.058.988169
CAGTGGT71962300.058.791064
GGTATCA72191650.058.617968
GTGGTAT72372950.058.4409986
TGGTATC72416100.058.4123157
GCAGTGG72932400.057.9786573
CGATAGG218950.022.78039735-39
CGACCTA239350.020.76017645-49
ATGGGGC2867900.020.2901825-29
CATGGGG8915900.019.81646325-29
ATGGGAT3281600.019.78057925-29
TACGCAC257750.019.31783750-54
ATGGGGT1995800.019.19465325-29
TGCGATA225450.019.0621330-34
GGTCGCG458100.017.94766650-54
TAACCCG152800.017.733644115-119
CGGCTAT249850.017.720394140-144
Produced by FastQC (version 0.11.5)